What is Tris-HCl Buffer, pH 7.0 used for?

Tris-HCl Buffer, pH 7.0 is commonly used in biological and biochemical applications. It helps to maintain a stable pH environment that is essential for enzyme activity, DNA/RNA electrophoresis, and protein purification. The Tris buffer effectively minimizes pH fluctuations, allowing researchers to achieve more reproducible results in experiments. For example, in molecular biology experiments, a stable pH is crucial during reactions such as PCR or gel electrophoresis, where variations could lead to distorted or inaccurate results.

How do you prepare Tris-HCl Buffer at pH 7.0?

To prepare Tris-HCl Buffer at pH 7.0, you need to dissolve Tris base in distilled water and adjust the pH with hydrochloric acid (HCl) to the desired level. The buffering capacity will depend on the concentration of Tris and the final volume. For practical purposes, pre-made 1M solutions like Tris-HCl Buffer, pH 7.0, provide convenience and consistency, especially in labs where precise pH values are critical. This method is routinely applied in research facilities for applications that require consistent buffering capacity.

Can Tris-HCl Buffer be used in protein assays?

Yes, Tris-HCl Buffer, pH 7.0, is widely used in protein assays. Its neutral pH is compatible with many proteins, which ensures their stability during analysis. For instance, it is remarkable for techniques like Western blotting and ELISA, where protein stability and enzymatic activity are paramount. Using the buffer prevents denaturation and maintains the structural integrity of proteins throughout the assay process, making it a go-to solution for researchers working on enzyme-linked studies.

Is Tris-HCl Buffer suitable for electrophoresis?

Absolutely! Tris-HCl Buffer, pH 7.0, is specifically formulated for electrophoresis applications. The buffer provides optimal conditions for the migration of DNA and RNA through gel matrices during electrophoretic techniques. It also helps to maintain the stability of nucleic acids, ensuring that they do not degrade during the process. Often, labs rely on this buffer in both agarose and polyacrylamide gel electrophoresis, where a consistent pH is crucial for resolving nucleic acid fragments accurately.

What volume of Tris-HCl Buffer should I use in experiments?

The volume of Tris-HCl Buffer, pH 7.0, to use in experiments varies depending on the specific protocol you are following. Commonly, for reactions and assays, volumes of 50-200 µL are typically sufficient for small-scale experiments, while larger setups may require several milliliters. By referencing the specific requirements of your experiment, you can ensure you are using the appropriate buffer volume for optimal results, supporting consistent outcomes throughout various laboratory applications.

How should Tris-HCl Buffer be stored?

Tris-HCl Buffer, pH 7.0, should be stored at room temperature or in a cool place and kept tightly sealed to prevent contamination. It's advisable to protect it from extreme temperatures or direct sunlight. Under these conditions, the buffer can be stored for extended periods while maintaining its stability. This practice is particularly important in laboratory settings where consistent results are needed, ensuring that the buffer maintains its pH and efficacy for future experiments.

Can Tris-HCl Buffer be used for DNA or RNA storage?

Tris-HCl Buffer, pH 7.0, can indeed be used for storing DNA or RNA samples, as it helps to prevent degradation by providing a stable pH environment. Storing nucleic acids in this buffer allows for long-term preservation without compromising their integrity, making it ideal for sample management in molecular biology. This usage is especially beneficial in lab settings when preparing samples for further analysis or experimentation, where maintaining their quality is paramount.

What are the benefits of using a pre-made Tris-HCl Buffer solution?

Using a pre-made Tris-HCl Buffer, pH 7.0 streamlines laboratory processes by ensuring correct concentrations and pH values are achieved without requiring additional calculations or adjustments. This saves time and reduces the possibility of error in preparation, which is critical for experiments demanding precision. Pre-made buffers are particularly valuable in high-throughput scenarios or collaborative research, where multiple scientists rely on the same consistent buffer for their methodologies.

What are the common applications of Tris-HCl Buffer in research?

Tris-HCl Buffer, pH 7.0, is versatile and finds applications in various domains, such as molecular biology, biochemistry, and genetic research. Common uses include enzyme assays, protein purification, PCR, and nucleic acid gel electrophoresis. Its ability to maintain consistent pH makes it an essential reagent during experiments that demand precision, thus facilitating accurate interpretation of results and enhancing reproducibility across experiments or research laboratories.

Where can I buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL?

You can buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL from Ubuy in Armenia. Ubuy offers a convenient platform for purchasing various laboratory supplies, including specialized buffers like Tris-HCl. Their inventory ensures that you can find quality products delivered directly to your location, catering to the needs of researchers and laboratory professionals looking for reliable reagents.

Как покупать Tris-HCl Buffer, pH 7.0 [1M] - 250mL в Интернете на Ubuy?

Купить Tris-HCl Buffer, pH 7.0 [1M] - 250mL онлайн на Ubuy очень просто.. Вам просто нужно найти товар, выбрать способ доставки при оформлении заказа и получить его до вашего места.

Доступен ли Tris-HCl Buffer, pH 7.0 [1M] - 250mL для покупок в Интернете в Armenia?

Да, на Ubuy Armenia вы можете купить этот товар по разумной цене.. Tris-HCl Buffer, pH 7.0 [1M] - 250mL недоступен на месте, но вы можете доверить нам наши услуги экспресс-доставки.

Сколько времени занимает получение товара после размещения заказа?

Время доставки заказанного вами товара зависит от того, что вы заказали, и выбранного вами способа доставки.. Ориентировочное время доставки указывается в процессе оформления заказа, так что будьте беззаботны при совершении покупок.

eCon Lab Supply Посмотреть оригинал

Manufactured in an ISO 9001 and 13485 certified facility in the USA under stringent Good Manufacturing Practices.

eCon Lab Supply

товар №: 97963856

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

98% of respondents would recommend this to a friend

товар №: 97963856

AMD 58653

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from США}

Доступность : В наличии

Импортировано из магазина USA

QTY:

Закажите сейчас и получите товар приблизительно Monday, Октябрь 05

Secured transaction

Our Top Logistics Partners

Manufactured in an ISO 9001 and 13485 certified facility in the USA under stringent Good Manufacturing Practices.

Гарантия U-Care:

Нет

Выберите план

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of США store (110-120). Recommended power converters Купите сейчас.

What Stands Out

Optimal pH Stability

This Tris-HCl buffer maintains a consistent pH of 7.0, ideal for biochemical assays and molecular biology applications, ensuring reliable experimental conditions and reproducible results.

High Concentration

At 1M concentration, this buffer solution offers a robust capability to resist pH changes, making it suitable for various intricate laboratory procedures involving sensitive reactions.

Convenient Volume Size

Available in a 250mL size, this buffer is perfect for regular laboratory use, minimizing waste while providing ample quantity for multiple experiments or applications.

Информация о продукте

Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.42
Item Weight	1.8 pounds (820 grams)
Brand	eCon Lab Supply

What's in the box

Bottle

Who Should Buy?

Biochemistry Students
Ideal for laboratory experiments involving enzyme reactions and protein studies at a controlled pH level.
Molecular Biology Research
Essential for applications like PCR, gel electrophoresis, and DNA/RNA manipulation requiring stable pH.
Clinical Labs
Useful in diagnostic tests that require precise buffer solutions for maintaining biological sample integrity.

Field Research
Not suitable for outdoor experiments due to stability issues and potential for buffer degradation over time.

ОПИСАНИЕ ТОВАРА

Есть вопрос? Пообщайтесь с нами в чате

Вопросы и ответы клиентов

вопрос: Как покупать Tris-HCl Buffer, pH 7.0 [1M] - 250mL в Интернете на Ubuy?

отвечать: Купить Tris-HCl Buffer, pH 7.0 [1M] - 250mL онлайн на Ubuy очень просто.. Вам просто нужно найти товар, выбрать способ доставки при оформлении заказа и получить его до вашего места.
вопрос: Доступен ли Tris-HCl Buffer, pH 7.0 [1M] - 250mL для покупок в Интернете в Armenia?

отвечать: Да, на Ubuy Armenia вы можете купить этот товар по разумной цене.. Tris-HCl Buffer, pH 7.0 [1M] - 250mL недоступен на месте, но вы можете доверить нам наши услуги экспресс-доставки.
вопрос: Сколько времени занимает получение товара после размещения заказа?

отвечать: Время доставки заказанного вами товара зависит от того, что вы заказали, и выбранного вами способа доставки.. Ориентировочное время доставки указывается в процессе оформления заказа, так что будьте беззаботны при совершении покупок.

eCon Lab Supply Buffers Editorial Review

Редакционных отзывов не найдено

Customer Reviews & Ratings

5.0

1 оценки клиентов

5 звезда

100%
4 звезда

0%
3 звезда

0%
2 звезда

0%
1 звезда

0%

Оцените этот товар

Поделитесь своими впечатлениями

Product Price History

Важная информация

Ограничения: обратите внимание, что для товаров, поставляемых за границу, гарантия производителя может быть недействительной; обслуживание от производителя может быть недоступно; руководства по эксплуатации, а также инструкции и предупреждения о безопасности могут быть не на языках страны назначения; товары (и сопутствующие материалы) могут не быть разработаны в соответствии со стандартами, спецификациями и требованиями к маркировке страны назначения; товары также могут не соответствовать напряжению в стране назначения и другим электрическим стандартам (при необходимости требуется использование адаптера или преобразователя). Получатель несет ответственность за обеспечение законного ввоза товара в страну назначения. При заказе через Ubuy или его аффилированных лиц получатель является зарегистрированным импортером и должен соблюдать все законы и правила страны назначения.
Не все товары на Ubuy выставлены на продажу, поскольку Ubuy — это глобальная поисковая система. На товары распространяются законы в области экспорта и торговли.

AMD 58653

Закажите сейчас и получите товар приблизительно Monday, Октябрь 05

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Особенности и преимущества

Comprehensive range of biological buffers for research and diagnostics.
Produced in an ISO certified facility ensuring quality.
Aseptically filtered for purity and reliability.
Suitable for protein research and molecular biology applications.
Complete Certificate of Analysis provided for each lot.
Dilutable Tris-HCl buffer for tailored concentration needs.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

AMD 58653

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Информация о продукте

What's in the box

Who Should Buy?

ОПИСАНИЕ ТОВАРА

Вопросы и ответы клиентов

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Оцените этот товар

Product Price History

Важная информация

AMD 58653

Особенности и преимущества

Ubuy Assurance

Fast
Shipping

Free
Return^*